Journal: Purinergic signalling

Article Title: Adenosine enhances cisplatin sensitivity in human ovarian cancer cells.

doi: 10.1007/s11302-018-9622-7

Figure Lengend Snippet: Fig. 6 Effect of SLV320, PSB603, and dipyridamole on the induction of apoptosis by adenosine. a Effect of 10 μM dipyridamole, 3 μM PSB603, and 3 μM SLV320 on the ability of 1000 μM adenosine to induce apoptosis over 48 h. b Increased effect of apoptosis induction by combination of adenosine (500 μM) and cisplatin (3-fold IC50). Adenosine was incubated for 48 h prior to addition of cisplatin for 24 h. Dipyridamole significantly decreased the effect of adenosine. c The com- bination of 500 μM adenosine 48 h prior to 3-fold IC50 of cisplatin for

Article Snippet: Adenosine, dipyridamole, and cisplatin were purchased from Sigma-Aldrich (Germany), and SCH442416, SLV320, and PSB603 were purchased from Tocris Bioscience (Germany).

Techniques: Incubation

Journal: International Journal of Molecular Sciences

Article Title: Ivy Leaf Dry Extract EA 575 ® Has an Inhibitory Effect on the Signalling Cascade of Adenosine Receptor A 2B

doi: 10.3390/ijms241512373

Figure Lengend Snippet: Influence of EA 575 ® ( A , B ) or the antagonists PSB-603 and SCH 442416 ( C ) on the CRE activation in transiently transfected HEK cells mediated by stimulation with adenosine ( A , C ) or BAY 60-6583 ( B ). Pre-incubation with 40–240 µg/mL EA 575 ® was conducted for 16 h ( A , B ) or with 0.1–10 µM of the antagonists for 2 h ( C ) before cells were stimulated by adding 100 µM adenosine ( A , C ) or 10 µM BAY 60-6583 ( B ). The non-specifically mediated CRE activation was significantly inhibited by pre-incubation with 80–240 µg/mL EA 575 ® ( A ) or 1 µM PSB-603 ( C ), compared to stimulated control cells not pre-incubated with EA 575 ® (SC). SCH 442416 also significantly reduced the CRE activation at a concentration of 10 µM, whereas this effect could not be observed at lower concentrations of both antagonists. Instead, 0.1 µM SCH 442416 slightly increased the CRE activation ( C ). The inhibition of the specific A 2B AR-mediated CRE activation was achieved by pre-incubation with 80–240 µg/mL EA 575 ® ( B ). Influence of the A 2A AR agonist CGS 21680 and, in comparison, BAY 60-6583 and adenosine on the CRE activation in transiently transfected HEK cells ( D ). Stimulation was performed with 1–10 µM CGS 21680, 10 µM BAY 60-6583, or 100 µM adenosine. The A 2A AR-mediated CRE activation was significantly increased by 10 µM CGS 21680 compared with completely untreated control cells (UTC), but to a lesser extent than that mediated by A 2B AR or non-specifically using adenosine ( D ). Data are shown as AUC of the fold change (FC) after stimulation normalised to stimulated control cells not pre-incubated with EA 575 ® (SC) ( A – C ) or completely untreated control cells (UTC) ( D ). Results represent the mean and SEM ( n = 3 independent experiments performed in triplicate, * p < 0.05).

Article Snippet: PSB-603 and CGS 21680 were obtained from Biomol (Hamburg, Germany).

Techniques: Activation Assay, Transfection, Incubation, Concentration Assay, Inhibition

Journal: International Journal of Molecular Sciences

Article Title: Ivy Leaf Dry Extract EA 575 ® Has an Inhibitory Effect on the Signalling Cascade of Adenosine Receptor A 2B

doi: 10.3390/ijms241512373

Figure Lengend Snippet: Influence of EA 575 ® ( A , B ) or the antagonists PSB-603 and SCH 442416 ( C ) on the IL-6 release of Calu-3 cells mediated by stimulation with adenosine ( A , C ) or BAY 60-6583 ( B ). Pre-incubation with 40–240 µg/mL EA 575 ® was conducted for 16 h ( A , B ) or with 0.1–10 µM of the antagonists for 1 h ( C ) before cells were stimulated by adding 100 µM adenosine ( A , C ) or 10 µM BAY 60-6583 ( B ) for another 24 h. The non-specifically mediated IL-6 release was significantly and dose-dependently inhibited by pre-incubation with 80–240 µg/mL EA 575 ® (A) or 1 µM PSB-603 ( C ), compared to stimulated control cells not pre-incubated with EA 575 ® (SC). SCH 442416, however, increased the concentration of IL-6 even further. ( C ). The inhibition of the specific A 2B AR-mediated IL-6 release was achieved by pre-incubation with 40–240 µg/mL EA 575 ® ( B ). Influence of the A 2A AR agonist CGS 21680 and, in comparison, BAY 60-6583 on the IL-6 release of Calu-3 cells ( D ). Stimulation was performed with 1–10 µM CGS 21680 or 10 µM BAY 60-6583 for 24 h. The A 2A AR-mediated IL-6 release was slightly, but neither significantly nor dose-dependently, increased compared to completely untreated control cells (UTC) ( D ). Results represent the mean normalised to stimulated control cells not pre-incubated with EA 575 ® (SC) ( A – C ) or completely untreated control cells (UTC) ( D ) and SEM ( n = 3 independent experiments performed in triplicate, * p < 0.05).

Article Snippet: PSB-603 and CGS 21680 were obtained from Biomol (Hamburg, Germany).

Techniques: Incubation, Concentration Assay, Inhibition